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Antigens provided by customers Antigens provided by customers should be soluble, not toxic, not infectious and at least 80% pure judged by SDS-PAGE. Molecular mass of antigens should preferably be over 20 kDa. Customers may provide us with fusion proteins. Small peptides or organic molecules need to be cross-linked onto carrier proteins first. The amount of antigens required is listed below.
We offer antigen expression services for customers who provide us with cDNA inserted expression vector for E. coli. We charge $700 US for 2-3 mg antigen expressed, which is sufficient for most monoclonal and polyclonal antibody production. Each of 3 mg additional antigen costs $300 US. The price includes transformation of the construct in E. coli, expression and purification of the antigen. Absea subcontracts peptide synthesis through specialist companies in China . Peptides are characterised by HPLC and mass spectroscopy. Absea provides antigenic peptides prediction from existing protein sequences free of charge. However, we do not guarantee that the peptide predicted will be immunogenic and antibodies raised against a peptide will recognise the native predicted protein. The costs for peptide synthesis must be settled before antibody generation can be initiated. Coupling peptides onto carrier proteins We normally use bovine serum albumin (BSA) and keyhole limpet hemocyanin (KLH) as carrier proteins. Peptides should contain cysteine residues. We charge $100 US for coupling 2-5 mg peptide onto BSA or KLH.
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