 |
 |
Antibody modification and characterisation
Antibody digestion to Fab
or F(ab')2 fragments
We use pepsin for F(ab')2 fragment production and papain for Fab fragment production. Before digesting large amount of antibodies, we perform an analytical digestion to optimise conditions. Undigested antibodies will be removed by protein A columns. Customers should provide minimum of 10 mg antibodies. Normally 3 mg of Fab or F(ab')2 fragments can be obtained from 10 mg antibodies after absorption with Protein A. The resulted fragments should remain their binding abilities to antigens and show one band (about 100 kDa for F(ab')2 fragments and 50 kDa for Fab fragments) under non-reducing conditions and two bands (both are around 25 kDa) on SDS-PAGE under reducing conditions. We charge $50 US for analytical digestion by pepsin and $100 for analytical digestion by papain. We charge $280 US for digestion of 10 mg antibody by pepsin and $350 US for digestion of 10 mg antibody by papain. Antibody labelling We charge $130 US for labelling peroxidase or FITC onto 5 mg of antibodies. Selection of antibodies for Western blotting and flow cytometry In monoclonal antibody production, positive cells are normally selected by ELISA. If customers require, we can go further to check antibody reactivates in Western blotting and flow cytometry. We make $100 US basic charge for screening up to 20 antibodies for Western blotting. For each positive antibody found, we charge additional $50 US. Providing cells are available, we make $150 US basic charge for screening up to 20 antibodies for flow cytometry. For each positive antibody found, we charge additional $50 US. Selection of capture and detection antibody pairs for sandwich ELISA Customers who require this service please contact us.
|
||
